Figure 5.
Directed evolution of the in silico designed retro-aldolase RA95. (a) The catalytic efficiencies of various RA95 variants with (R)-methodol. The data in all panels in this figure were compiled from Giger et al. [82] and Obexer et al. [83]. (b) Mutations introduced in succeeding rounds of directed evolution of RA95. (c) Positions of the introduced mutations in each round of directed evolution, projected on the crystal structure of RA95 (PDB ID: 4A29) [82].