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. 2018 Jul 12;19(7):2027. doi: 10.3390/ijms19072027

Figure 5.

Figure 5

Effects of ASP and ASPA on inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNA and protein expression. RAW 264.7 cells were treated with ASP and ASPA (40, 80, and 160 μg/mL) for 1 h and then induced with LPS (50 ng/mL) for 24 h. mRNA levels were analyzed by real-time PCR. (A) The levels of iNOS mRNA in ASP treatment groups. (B) The levels of COX-2 mRNA in ASP treatment groups. (C) The levels of iNOS mRNA in ASPA treatment groups. (D) The levels of COX-2 mRNA in ASPA treatment groups. The proteins were analyzed by Western blot. The quantitative evaluation of protein band by densitometry was shown. (E) The expression levels of iNOS and COX-2 in ASP treatment groups. (F) The expression levels of iNOS and COX-2 in ASPA treatment groups. * p < 0.05, ** p < 0.01 and *** p < 0.001 versus LPS-only treatment group; ### p < 0.001 versus control group (n = 3).