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. 2018 Jun 22;10(7):802. doi: 10.3390/nu10070802

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of fish oil on hepatic adiponectin receptor 2 (adipoR2), AMP-activated protein kinase-α (AMPKα), phosphorylated (p)-AMPKα, and NAD-dependent deacetylase sirtuin-1 (SIRT1) protein expressions in rats with chronic ethanol feeding. Western blots analysis of (a) adipoR2, (b) AMPKα, (c) p-AMPKα, and (d) SIRT1 protein expressions. β-Actin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. Quantitative analysis of protein levels and the ratio to each internal control was calculated by setting the value of group C as 1. Values are expressed as the mean ± SEM. An asterisk (*) shows a significant difference between C and E groups (p < 0.05). Means with different superscript letters shows a significant difference (a,b) among C, CF25, and CF57 groups (p < 0.05). Means with different superscript letters shows a significant difference (e,f) among E, EF25, and EF57 groups (p < 0.05).

Effects of fish oil on hepatic adiponectin receptor 2 (adipoR2), AMP-activated protein kinase-α (AMPKα), phosphorylated (p)-AMPKα, and NAD-dependent deacetylase sirtuin-1 (SIRT1) protein expressions in rats with chronic ethanol feeding. Western blots analysis of (a) adipoR2, (b) AMPKα, (c) p-AMPKα, and (d) SIRT1 protein expressions. β-Actin or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal control. Quantitative analysis of protein levels and the ratio to each internal control was calculated by setting the value of group C as 1. Values are expressed as the mean ± SEM. An asterisk (*) shows a significant difference between C and E groups (p < 0.05). Means with different superscript letters shows a significant difference (a,b) among C, CF25, and CF57 groups (p < 0.05). Means with different superscript letters shows a significant difference (e,f) among E, EF25, and EF57 groups (p < 0.05).