Table 5.
Effects of fish oil on hepatic fatty acid metabolism-related gene mRNA levels in rats with chronic ethanol feeding 1,2,3.
| mRNA Levels | F25 | F57 | Ethanol × Fish Oil | ||
|---|---|---|---|---|---|
| SREBP-1c | C | 1.00 ± 0.30 | 1.54 ± 0.29 | 1.90 ± 0.38 | 0.5486 |
| E | 1.55 ± 0.23 | 1.60 ± 0.17 | 1.92 ± 0.36 | ||
| FAS | C | 1.00 ± 0.29 | 1.48 ± 0.19 | 1.87 ± 0.50 | 0.2566 |
| E | 1.57 ± 0.27 | 1.50 ± 0.2 | 1.53 ± 0.12 | ||
| SCD-1 | C | 1.00 ± 0.22 | 1.34 ± 0.19 | 1.37 ± 0.22 | 0.7705 |
| E | 1.41 ± 0.29 | 1.48 ± 0.26 | 1.78 ± 0.19 | ||
| PPARα | C | 1.00 ± 0.28 b | 2.03 ± 0.24 a | 2.11 ± 0.28 a | 0.7043 |
| E | 1.39 ± 0.40 | 2.15 ± 0.42 | 2.03 ± 0.19 | ||
| MCAD | C | 1.00 ± 0.14 | 0.96 ± 0.14 | 1.20 ± 0.14 | 0.2107 |
| E | 0.83 ± 0.12 f | 1.13 ± 0.05 e,f | 1.63 ± 0.33 e | ||
| ACO1 | C | 1.00 ± 0.51 | 1.32 ± 0.15 | 1.25 ± 0.14 | 0.9118 |
| E | 0.93 ± 0.18 | 1.41 ± 0.32 | 1.40 ± 0.21 | ||
| CPT-1 | C | 1.00 ± 0.21 | 1.16 ± 0.17 | 1.37 ± 0.43 | 0.5085 |
| E | 0.99 ± 0.13 f | 1.09 ± 0.12 f | 1.74 ± 0.14 e |
1 Values are expressed as the mean ± SEM. Means with different superscript letters shows a significant difference (a,b) among C, CF25, and CF57 groups (p < 0.05). Means with different superscript letters shows a significant difference (e,f) among E, EF25, and EF57 groups (p < 0.05). 2 Details are the same as those described in Table 3. 3 Comparative quantification of each gene was normalized to β-actin using the 2−ΔΔCt method. SREBP, sterol response element-binding protein; FAS, fatty acid synthase; SCD, stearoyl coenzyme A desaturase; PPAR, peroxisome proliferator-activated receptor; MCAD, medium-chain acyl-coenzyme A dehydrogenase; ACO1, acyl-CoA oxidase 1; CPT, carnitine palmitoyl transferase.