Figure 2.

Incubation of TARDIS slides with 20S proteasomes. TARDIS slides prepared from etoposide-treated K562 cells were treated with 1 µg 20S proteasome preparations. After 90 min, remaining TOP2A-DNA covalent complexes were detected by quantitative immunofluorescence. All fluorescence values were normalised to the values obtained following exposure of cells to 100 µM etoposide and subsequent incubation in preparation buffer without 20S proteasomes. Data is presented as the normalised mean of medians ± SEM (histogram) and raw integrated fluorescence values from a single experiment before normalisation (scatter diagram).