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. 2018 Jul 14;19(7):2056. doi: 10.3390/ijms19072056

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cellular depletion of TDP2 does not affect topoisomerase II protein complex levels. TDP2 was depleted by two independent siRNAs. (A) Western blot visualising TDP2 protein levels after 48 and 72 h. (B,C) Cells transfected with 500 nM TDP2 siRNA #1 or non-coding (CON) siRNA were exposed to etoposide or solvent for 2 h and then placed in drug free media. TOP2A- and TOP2B-DNA complexes were quantified by TARDIS assay immediately after drug treatment and after 0.5, 1 and 2 h incubation in drug free media. Values are normalised to a 2 h 100 µM etoposide control (n = 6).

Cellular depletion of TDP2 does not affect topoisomerase II protein complex levels. TDP2 was depleted by two independent siRNAs. (A) Western blot visualising TDP2 protein levels after 48 and 72 h. (B,C) Cells transfected with 500 nM TDP2 siRNA #1 or non-coding (CON) siRNA were exposed to etoposide or solvent for 2 h and then placed in drug free media. TOP2A- and TOP2B-DNA complexes were quantified by TARDIS assay immediately after drug treatment and after 0.5, 1 and 2 h incubation in drug free media. Values are normalised to a 2 h 100 µM etoposide control (n = 6).