Skip to main content
. 2018 Jul 14;19(7):2056. doi: 10.3390/ijms19072056

Figure 5.

Figure 5

Effect of TDP2 siRNA knockdown on levels of SUMOylated TOP2-DNA complexes following proteasomal inhibition. (A) K562 cells were treated with 100 µM etoposide (or equivalent volume of DMSO) for 2 hours, and SUMOylated TOP2-DNA complexes were visualized using the TARDIS assay; (B) Control or TDP2 siRNA knockdown cells were treated with 100 µM etoposide and 10 µM MG132 for 2 h, followed by incubation in etoposide-free medium containing MG132 for 0, 0.5, 1, or 2 h. Levels of SUMOylated TOP2-DNA complexes were measured using the TARDIS assay. Values are normalised to a 2 h 100 µM etoposide control and compared by two-way ANOVA (n = 3).