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. 2018 Jun 21;19(7):1833. doi: 10.3390/ijms19071833

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Overexpression of S100A8/A9 in macrophages leads to increased IL-10 expression. (A–C) Validation of transfection efficiency of the THP-1 macrophage cell line. The following cytospins were inspected by immunofluorescence for green light emission: (A) non-transfected cells; (B) cells transfected with an empty plasmid; (C) and those with a GFP-containing plasmid. Scale bar: 50 µm. The results show high transfection efficiency in the cells; (D) Overexpression of S100A8 and S100A9 leads to a consistent increase of IL-10 expression, but not of TNFα and IL-1β. This was observed in both unstimulated cells (left panel) and LPS–stimulated cells (right panel). Data are shown as boxplots (median, upper value, lower value) representing three different experiments.