Figure 4.

O-glycosylation capacity affects mesothelial clearance of OVCAR3. (A) Representative images from mesothelial clearance assays of OVCAR3 (upper) and OVCAR3 SC (bottom), taken at 0 and 18 h of co-culture; (B) Quantification of mesothelial clearance in parental OVCAR3 and OVCAR3 SC with a significant decrease of mesothelial clearance in OVCAR3 SC. The clearance area was measured by co-culturing aggregates of ovarian cancer cells with MeT5A-EGFP mesothelial cell monolayers. After 18 h of co-culture, the negative space created in the mesothelial monolayer by the ovarian cancer aggregates was measured and divided by the initial size of the ovarian cancer aggregates at time 0 to determine the normalized clearance area. More than eight aggregates were scored over three independent experiments. Scale bar, 20 µm. Values represent the mean ± SD, **** p < 0.0001.