Table 2.
Assessment of total polyphenol, total flavonoid content and antioxidant activity of Geotrichum and Penicillium extracts. Data presents mean ± standard error. Different letters inside of parenthesis indicate the significant difference among the treatments at p < 0.05 using the Tukey’s HSD test.
| Geotrichum candidum | ||||||
| Extracts | Total Polyphenols (mg GAE/g) | Total Flavonoids (mg QE/g) | IC50 (µg/mL) | |||
| DPPH | β-carotene | RP | ABTS | |||
| EtOH | 28.93 ± 0.89 (ab) | 14.41 ± 0.16 (c) | 222.16 ± 3.08 (d) | 219.14 ± 1.02 (d) | 211.48 ± 0.37 (c) | 191.36 ± 0.41 (e) |
| EtOAc | 33.39 ± 0.49 (b) | 11.25 ± 1.03 (ab) | 177.55 ± 0.96 (b) | 162.86 ± 0.34 (b) | 190.3 ± 3.36 (b) | 151.31 ± 4.2 (c) |
| Hexane | 27.51 ± 2.78 (a) | 10.03 ± 0.27 (a) | 223.14 ± 2.71 (d) | 220.27 ± 2.07 (d) | 218.71 ± 3.24 (d) | 205.98 ± 8.35 (f) |
| MetOH | 31.77 ± 2.23 (ab) | 12.46 ± 0.6 (b) | 207.44 ± 1.48 (c) | 171.75 ± 1.08 (c) | 205.93 ± 0.41 (c) | 177.9 ± 1.71 (d) |
| BHT 1 | - | - | 34.33 ± 0.03 (a) | 50.11 ± 0.18 (a) | - | - |
| Trolox 2 | - | - | - | - | - | 132.98 ± 0.95 (b) |
| Ascorbic Acid | - | - | - | - | 182.08 ± 1.12 (a) | 116.75 ± 0.32 (a) |
| Penicillium citrinum | ||||||
| Extracts | Total Polyphenols (mg GAE/g) | Total Flavonoids (mg QE/g) | IC50 (µg/mL) | |||
| DPPH | β-carotene | RP | ABTS | |||
| EtOH | 18.87 ± 0.36 (a) | 5.11 ± 0.19 (d) | 268.4 ± 4.46 (d) | 252.84 ± 1.66 (c) | 272.49 ± 4.44 (c) | 266.4 ± 6.41 (e) |
| EtOAc | 21.24 ± 1.4 (ab) | 2.29 ± 0.06 (b) | 246.36 ± 0.45 (c) | 239.42 ± 1.71 (b) | 264.76 ± 4.43 (c) | 227.03 ± 1.37 (d) |
| Hexane | 16.69 ± 1.51 (a) | 1.41 ± 0.33 (a) | 370.8 ± 3.39 (e) | 264.55 ± 2.59 (d) | 301.78 ± 5.81 (d) | 277.02 ± 6.58 (f) |
| MetOH | 24.12 ± 3.36 (b) | 4.07 ± 0.08 (c) | 227.87 ± 0.49 (b) | 235.28 ± 1.15 (b) | 233.89 ± 3.32 (b) | 215.04 ± 1.31 (c) |
| BHT 1 | - | - | 34.33 ± 0.03 (a) | 50.11 ± 0.18 (a) | - | - |
| Trolox 2 | - | - | - | - | - | 132.98 ± 0.95 (b) |
| Ascorbic Acid | - | - | - | - | 182.08 ± 1.12 (a) | 116.75 ± 0.32 (a) |
| Positive control | ||||||
(mg GAE/g): mg of gallic acid equivalent per g of fungal extract; (mg QE/g): mg of quercitin acid equivalent per g of fungal extract; IC50 (µg/mL) values corresponding to the amount of the extract providing 50% of inhibition percentage. 1: 1,2,3-benzothiadiazole-7-carbothioic acid S-methyl ester; 2: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid.