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. 2018 Jul 20;19(7):2118. doi: 10.3390/ijms19072118

Figure 6.

Figure 6

SIRT1 expression and activity in ARPE-19 cells under inflammatory condition. (a) Analysis of gene expression showed that treatment with 10 µg/mL LPS for 24 h downregulated SIRT1 expression level (FC = 0.57; p = 0.003). (b) Analysis of SIRT1 enzymatic activity using a fluorimetric assay confirmed that total SIRT1 activity was reduced by 20.1% in LPS-treated cells compared to controls (p = 0.002). Treatment with 10 μM resveratrol for 24 h restores SIRT1 expression (FC = 1.04, p = 0.009) and activity (105.5%, p = 0.005) in cells under inflammatory condition. The experiments were performed in triplicate and repeated three times. Bar graphs show mean ± SE. ** p < 0.01 based on the Student’s t-test.