Table 2.
Oligonucleotide primers used in this study.
| Primer Designation a | Sequence (5′ to 3′) |
|---|---|
| OTT_RS06375-1F | ATGATTAAAAAGTCAATTATTAGTATATGTGTATTAGTGC |
| OTT_RS06375-615R | CTATGCTATATTACTTTTAATAATTGTGACAGACC |
| OTT_RS06375-64F | TGTTTATGGCAAAGATCTAAACATAGTAAC |
| ompA-57F | GTGGAAATGTTTATGGCAAAGATCTAAAC |
| ompA-260R | GCTTGTAAAAACTGTTCATGCTTTACATC |
| Eubacterial 16S-F | GTTCGGAATTACTGGGCGTA |
| Eubacterial 16S-R | AATTAAACCGCATGCTCCAC |
| R17K-135 | ATGAATAAACAACGKbCANGGHACAC |
| R17K-249 | RAAGTAATGCRCCTACACCTACTC |
a F and R refer to primers that bind to the sense and antisense strand, respectively. The number immediately preceding the F or R denotes the first nucleotide position where the primer binds. b Degenerate positions contained equal molar base concentrations of the following nucleotides: (K), guanine and thymine, (N), adenine, guanine, thymine, and cytosine; (H), adenine, guanine, and thymine; (R) adenine and guanine.