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. 2018 Jul 13;10(7):901. doi: 10.3390/nu10070901

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Inhibitory effect PLE0 on RANKL-induced expression of c-Fos and NFATc1 in BMMs. (A,B) BMMs were treated with PLE0 (25 mg/mL, -●-) or vehicle (-○-) in the presence of M-CSF (60 ng/mL) and RANKL (100 ng/mL) on the various days of incubation. (A) Whole-cell extracts were subjected to Western blot analysis with specific antibodies as indicated; (B) Total RNA was isolated at the indicated time points and mRNA expression of NFATc1, c-Fos, TRAP, ATPv0d2, cathepsin K, and DC-STAMP was analyzed using real-time quantitative PCR. PCR data are represented as mean ± SEM and were analyzed with a one-way analysis of variance (ANOVA) test and Dunnett’s post hoc test. *, p < 0.05 versus no PLE0 treatment. **, p < 0.01 versus no PLE0 treatment. ***, p < 0.001 versus no PLE0 treatment.