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Epigenetic regulation of ATP1A1-AS1 expression in HK2 cells. Cultured HK2 cells were treated with deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA) (A) or DNA methylation inhibitor Decitabine (B) for 48 h and the total RNA was extracted using a commercial RNA extraction kit as described in Method section. The expression of ATP1A1-AS1-203 and ATP1A1 was measured using RT-qPCR. These experiments were repeated four times. Data was analyzed using Two-way ANOVA.
