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. 2018 Jul 21;19(7):2123. doi: 10.3390/ijms19072123

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Overexpression of ATP1A1-AS1-203 negatively regulates ATP1A1 gene expression and Na/K-ATPase α1 protein level in HK2 cells. HK2 cells were transiently transfected with pcDNA3.1(-B) plasmid that overexpresses ATP1A1-AS1-203 (AS3) or empty vector (EV) for 48 h and expression of ATP1A1-AS1-203 (A) and ATP1A1 (B) were measured using RT-qPCR. The Na/K-ATPase α1 protein level (C) was measured by Western blot using the Radioimmunoprecipitation assay (RIPA) buffer resolved cell lysates. These experiments were repeated 4 times. Data was analyzed using t-test.