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. 2018 Jul 21;19(7):2123. doi: 10.3390/ijms19072123

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of intron 2 of ATP1A1-AS1-203 on the expression of ATP1A1 in HK2 cells. (A) The expression level of ATP1A1-AS1-203 and ATP1A1-AS1-203-Intron 2 in HK2 cells; (B,C) The effect of ATP1A1-AS1-203-Intron 2 overexpression on ATP1A1 gene expression. The ATP1A1-AS1-203-Intron 2 overexpression vector (Intron2) was transiently transfected into HK2 cells for 48 h and the total RNA extracted from the cell lysates were used for RT-qPCR measurement of ATP1A1-AS1-203-Intron 2 (B) and ATP1A1 (C). These experiments were repeated four times. Data was analyzed using t-test.