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. 2018 Jul 21;19(7):2123. doi: 10.3390/ijms19072123

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Overexpression of ATP1A1-AS1-203 inhibits ouabain-induced Src activation in HK2 cells. HK2 cells were transfected with ATP1A1-AS1-203 overexpressing vector (AS3) or empty vector (EV) for 24 h followed by ouabain (Oua) treatment for 15 min. Cell lysates were collected in RIPA buffer. The phospho-Src at Tyr418 (pSrc418) and c-Src were probed using Western blot. The upper panel was a representative Western blot, and the lower panel was the quantification result. These experiments were repeated four times. Data was analyzed using Two-way ANOVA.