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. 2018 Jul 21;19(7):2123. doi: 10.3390/ijms19072123

Figure 8.

Figure 8

Overexpression of ATP1A1-AS1-203 inhibits HK2 cell growth in presence or absence of ouabain. HK2 cells were transfected with ATP1A1-AS1-203 vector (AS3) or empty vector (EV) for 48 h, followed by treatment of 50 nM ouabain (Oua) for another 48 h. Cells without ouabain treatment was used as control. Cell proliferation was monitored using an Incucyte incubator equipped with a camera that automatically taking pictures every 2 h at 9 different regions of each cell culture well. (A) Cell growth curve in AS3 or EV transfected HK2 cells without ouabain treatment; (B) Cell growth curve in AS3 or EV transfected HK2 cells that treated with 50 nM ouabain. These experiments were repeated four times. Data was analyzed using Two-way ANOVA.