Figure 3.

Knock-down of ITGB2-AS1 will exert inhibitory effect on migration and invasion of breast cancer cell. (A) The efficacy of siRNA fragment was verified by quantitative PCR; (B) The cell viability was detected by MTT assay; (C) The cell cycle distribution was measured by flow cytometry. Left panel: X-axis: cell number; Y-axis: DNA content; (D) The migration was evaluated by wound healing assay; (E) The invasion was detected by transwell chamber assay. Stained with 0.1% crystal violet (400×). Data are shown as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control groups.