Figure 5.

Overexpression of ITGB2 also could facilitate the migration and invasion of MCF-7 cell. (A) The verification of ITGB2 plasmid with western blotting assay; the β-actin was used as internal reference; (B) The cell cycle was evaluated by flow cytometry. Left panel: X-axis: cell number; Y-axis: DNA content; (C)The migration was detected by wound healing assay; (D) The cell invasion measured by transwell chamber assay, stained by 0.1% crystal violet (400×); (E) The detection of FAK signaling and MMP9 protein by western blotting assay. * p < 0.05, *** p < 0.001 vs. control groups.