Table 10.
Gene | Accesion No. | Primer Source | Primer Sequence (5′→3′) | Orientation | Tm (°C) | Amplicon Lenght (bp) | References |
---|---|---|---|---|---|---|---|
IL-1β | NM_214055 | Pig | ATGCTGAAGGCTCTCCACCTC | forward | 62 | 89 | [54] |
TTGTTGCTATCATCTCCTTGCAC | reverse | 59 | |||||
TLR2 | NM_213761.1 | Pig | TCACTTGTCTAACTTATCATCCTCTTG | forward | 59 | 162 | [55] |
TCAGCGAAGGTGTCATTATTGC | reverse | 59 | |||||
TLR4 | NM_001113039.1 | Pig | GCCATCGCTGCTAACATCATC | forward | 60 | 108 | [55] |
CTCATACTCAAAGATACACCATCGG | reverse | 59 | |||||
TLR6 | NM_213760.1 | Pig | AACCTACTGTCATAAGCCTTCATTC | forward | 59 | 95 | [55] |
GTCTACCACAAATTCACTTTCTTCAG | reverse | 59 | |||||
IL-10 | NM_214041.1 | Pig | GGCCCAGTGAAGAGTTTCTTTC | forward | 54 | 55 | [56] |
CAACAAGTCGCCCATCTGGT | reverse | 51 | |||||
IL-4 | NM_214123.1 | Pig | CAACCCTGGTCTGCTTACTG | Forward | 52 | 173 | [57] |
CTTCTCCGTCGTGTTCTCTG | reversed | 52 | |||||
Cyclophilin A | NM_214353.1 | Pig | CCCACCGTCTTCTTCGACAT | forward | 54 | 92 | [58] |
TCTGCTGTCTTTGGAACTTTGTCT | reverse | 55 | |||||
β-actina | NM_213978.1 | Pig | GGACTTCGAGCAGGAGATGG | forward | 60 | 230 | [59] |
GCACCGTGTTTGCGTAGAGG | reverse | 62 |