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. 2018 Jun 30;19(7):1923. doi: 10.3390/ijms19071923

Table 10.

Oligonucleotide Polymerase chain reaction (PCR) primers.

Gene Accesion No. Primer Source Primer Sequence (5′→3′) Orientation Tm (°C) Amplicon Lenght (bp) References
IL-1β NM_214055 Pig ATGCTGAAGGCTCTCCACCTC forward 62 89 [54]
TTGTTGCTATCATCTCCTTGCAC reverse 59
TLR2 NM_213761.1 Pig TCACTTGTCTAACTTATCATCCTCTTG forward 59 162 [55]
TCAGCGAAGGTGTCATTATTGC reverse 59
TLR4 NM_001113039.1 Pig GCCATCGCTGCTAACATCATC forward 60 108 [55]
CTCATACTCAAAGATACACCATCGG reverse 59
TLR6 NM_213760.1 Pig AACCTACTGTCATAAGCCTTCATTC forward 59 95 [55]
GTCTACCACAAATTCACTTTCTTCAG reverse 59
IL-10 NM_214041.1 Pig GGCCCAGTGAAGAGTTTCTTTC forward 54 55 [56]
CAACAAGTCGCCCATCTGGT reverse 51
IL-4 NM_214123.1 Pig CAACCCTGGTCTGCTTACTG Forward 52 173 [57]
CTTCTCCGTCGTGTTCTCTG reversed 52
Cyclophilin A NM_214353.1 Pig CCCACCGTCTTCTTCGACAT forward 54 92 [58]
TCTGCTGTCTTTGGAACTTTGTCT reverse 55
β-actina NM_213978.1 Pig GGACTTCGAGCAGGAGATGG forward 60 230 [59]
GCACCGTGTTTGCGTAGAGG reverse 62