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. 2018 Jul 12;19(7):2025. doi: 10.3390/ijms19072025

Figure 4.

Figure 4

AITC positively regulates MUC2 expression and attenuates DSS-induced mucin depletion in the LS174T cell line and in mice. To evaluate mucin expression, Western blot analysis using the MUC2 specific antibody and PAS staining were performed (PAS staining, mucin: magenta color). (A) AITC treatment (1, 5, or 10 μM) for 12 h increased MUC2 in LS174T (Right, 200× magnification); (B) AITC pretreatment ameliorated MUC2 decrease by DSS in LS174T (Right, 200× magnification); and (C) Mucin and goblet cell depletion in DSS-induced colitis was protected by AITC treatment. Colon tissues were stained with PAS staining (Scale bar 100 μm).