Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 21;19(7):2122. doi: 10.3390/ijms19072122

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Markers used to identify hematopoietic progenitors display graded, rather than discontinuous, expression. CD117, Sca-1 and Flt3 show a continuous expression making the identification of cells with “high” or “intermediate” expression of markers almost arbitrary. Figure shows a typical gating strategy for the identification of LSK (red), LMPP (green) and CLP (blue) progenitor populations based on the expression of CD117, Sca-1, Flt3 and CD127 (IL-7Rα). LSK are identified as Lineage^-CD117^highSca-1^high [2] and LMPP as the LSK with the highest expression of Flt3 [74]. CLP are gated as Lineage^-CD117^intSca-1^intFlt3⁺CD127⁺ [75,76]. LSK: Lineage-negative, Sca-1-positive, kit-positive cells; LMPP: Lymphoid-primed Multi-Potent Progenitors; CLP: Common Lymphoid Progenitors.