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. 2018 Jun 26;19(7):1868. doi: 10.3390/ijms19071868

Figure 1.

Figure 1

The proliferation of mesenchymal stem/stromal cells from adipose tissue (Ad-MSCs) varies depending on the donor site. Ad-MSCs derived from adipose tissue of the knee (N = 12), limb (N = 11), abdomen (N = 11), thigh (N = 12), and hip (N = 12) were osteogenically differentiated for 14 days. (a) To determine cell proliferation, the total protein content was determined by Sulforhodamine B (SRB) staining on days 0 and 14 of differentiation; (b) Relative cell numbers on day 14 of differentiation as a function of the donor age; (c) Relative cell numbers on day 14 of differentiation as a function of the donor body mass index (BMI); (d) Comparison of the relative cell numbers of differentiated (day 14) Ad-MSCs from male and female donors; °° p < 0.01 and °°° p < 0.001 when comparing day 0 with day 14 within each group; * p < 0.05, ** p < 0.01, and *** p < 0.001 as indicated.