Figure 4.

The expression of Runx2 and SP7 in Ad-MSCs varies depending on the donor site. Ad-MSCs derived from adipose tissue of the knee, limb, abdomen, thigh, and hip (N = 4 each) were osteogenically differentiated for 14 days. The relative expression levels of (a) Runx2 and (b) SP7 were determined by semi-quantitative RT-PCR. The PCR products were loaded twice (n = 2) to minimize the loading errors. The signal intensities were quantified with the help of the ImageJ software; (c) Representative images for the RT-PCR products from the pooled samples; (d) Representative images for the Western blot signals obtained from the pooled samples; * p < 0.05 and ** p < 0.01 as indicated.