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. 2018 Jun 26;19(7):1868. doi: 10.3390/ijms19071868

Figure 5.

Figure 5

The expression of stem cell factors in Ad-MSCs varies depending on the donor site. The expression levels of the stem cell factors (a) Oct4α, (b) Lin28A, (c) Nanog, and (d) Sox2 were determined by semi-quantitative RT-PCR in Ad-MSCs derived from adipose tissue of the knee, limb, abdomen, thigh, and hip (N = 4 each). The PCR products were loaded twice (n = 2) to minimize the loading errors. The signal intensities were quantified with the help of the ImageJ software. (e) Representative images for the RT-PCR products from the pooled samples; (f) Representative images for the Western blot signals obtained from the pooled samples; * p < 0.05, ** p < 0.01, and *** p < 0.001 as indicated.