Table 2.
PCR conditions.
| Gene | GeneBank ID [NM] | Forward Primer | Reverse Primer | Ta [°C] | Amplicon [bp] |
|---|---|---|---|---|---|
| Runx2 | 001015051 | TGCCTAGGCGCATTTCAGGTGC | GGTGGTGGTGCATGGCGGAA | 58 | 359 |
| SP7 | 152860 | CCCAGGCAACACTCCTACTC | GGCTGGATTAAGGGGAGCAAA | 58 | 175 |
| Oct4a | 002701.4 | AGTGAGAGGCAACCTGGAGA | GCCTCAAAATCCTCTCGTTG | 59 | 180 |
| Lin28A | 024674.4 | CCGAACCCCATGCGCACGTT | TTTGCAGGTGGCTGCGCCAAG | 59 | 137 |
| Nanog | 024865.2 | AACTGGCCGAAGAATAGCAA | ACTGGATGTTCTGGGTCTGG | 59 | 175 |
| Sox2 | 003106.3 | CATGCACCGCTACGACG | CGGACTTGACCACCGAAC | 62 | 152 |
| GAPDH | 002046.4 | GTCAGTGGTGGACCTGACCT | AGGGGTCTACATGGCAACTG | 56 | 420 |
Ta—annealing temperature.