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. 2018 Jul 9;19(7):2004. doi: 10.3390/ijms19072004

Figure 3.

Figure 3

Inhibition of EGF-induced ERK phosphorylation and cyclin D1 expression by anti-hMUC1 monoclonal antibody. (a) Capan-2 and (b) PANC-1 cells were pretreated with 10 µg/mL anti-hMUC1 monoclonal antibody (anti-hMUC1 Ab) for 6 h followed by stimulation with 10 ng/mL EGF for 5 min. Cell lysates were separated by SDS-PAGE and western blot analysis was performed with anti-phospho-ERK, anti-ERK and anti-β-actin antibodies. (c) Capan-2 cells and (d) PANC-1 cells were treated with 10 µg/mL anti-hMUC1 monoclonal antibody for 6 h followed by treatment with 10 ng/mL EGF and incubated for 24 h. Cell lysates were separated by SDS-PAGE and western blot analysis was performed with anti-cyclin D1 and anti-β-actin antibodies. These results are representatives of three independent experiments.