Figure 3.

Assessment of exercise (EX) effects on superoxide, endothelial nitric oxide synthase (eNOS), and glutathionylation after sham (SH), myocardial infarction (MI), and transverse aortic constriction (TAC). (A) Lucigenin-enhanced chemiluminescence superoxide production is expressed as relative light unit (RLU) per second per gram protein; (B) evaluation of superoxide production with N^G-nitro-L-arginine methyl ester (L-NAME), presented as L-NAME-independent superoxide; (C) subtraction of L-NAME-independent superoxide from total superoxide, presented as L-NAME-inhibitable superoxide; (D) eNOS protein expression; (E) phosphorylated eNOS (p-eNOS) serine (Ser)1177 protein expression; (F) eNOS monomer–dimer ratio; (G) oxidized glutathion (GSSG) and reduced glutathion (GSH) ratio; (H) S-glutathionylation of eNOS; (I) superoxide dismutase (SOD) activity; (J) representative western blot of eNOS protein expression; (K) representative western blot of p-eNOS serine (Ser)1177 protein expression; (L) representative eNOS monomer dimer blot; (I) representative co-immunoprecipitation of eNOS S-glutathionylation with unspecific mouse immunoglobulin G (IgG) antibody as a negative control. p-eNOS: phosphorylated eNOS; eNOS S-glut: eNOS S-glutathionylation. * p < 0.05 vs. corresponding sham, † p < 0.05 vs. corresponding sedentary. n = 3–8 in all groups.