Table 4.
Real-time PCR primers sequence.
| Gene | Sequence | Annealing Temperature (°C) | Aim Band Length (bp) |
|---|---|---|---|
| TCONS_00013557 | F 5′ GCTGCTGCCCTTGGACCTT 3′ | 60 | 58 |
| TCONS_00013557 | R 5′ CGTCACCCACAAACAGAGCA 3′ | ||
| Osr2 (XM_008255788.2) | F 5′ GCACACCCAGACCTCGCCG 3′ | 60 | 101 |
| Osr2 (XM_008255788.2) | R 5′ AACAACACGTAGAAAATAGCCCG 3′ | ||
| Col2a1 (XM_002723439.3) | F 5′ CATGAGGGCGCGGTAGAGA 3′ | 60 | 193 |
| Col2a1 (XM_002723439.3) | R 5′ CTTTGGTCCTGGTTTCCGG 3′ | ||
| Col11a1 (XM_017346047.1) | F 5′ CTGGATCCAATGAGATAAATGGC 3′ | 60 | 104 |
| Col11a1 (XM_017346047.1) | R 5′ CCCTGGTGGTCCTTCAACAA 3′ | ||
| XR_518424.2 | F 5′ ACCCTAGTAATTCAGCCTGCTCT 3′ | 60 | 140 |
| XR_518424.2 | R 5′ TGAGTGGTGAGGGAATGGAATA 3′ | ||
| Vgll2 (XM_008263422.2) | F 5′ TCAGCGTGGACTCAGCTCGT 3′ | 60 | 135 |
| Vgll2 (XM_008263422.2) | R 5′ CACGAAGTGAGAGGCACAGATG 3′ | ||
| Cav1 (XM_008258165.2) | F 5′ TGGGAACGACCTGAGGGTG 3′ | 60 | 56 |
| Cav1 (XM_008258165.2) | R 5′ AGTGTAGAGATGTCCCTGCACCA | ||
| Cav1 (XM_008258166.2) | F 5′ TGAGCGGCCGCTGTCGA 3′ | 60 | 113 |
| Cav1 (XM_008258166.2) | R 5′ ACTTGCTTCTCGTTCACCTCG 3′ | ||
| Hoxd10 (NM_001206424.1) | F 5′ AAGGAAAGCAAAGAGGAAATCAAG 3′ | 60 | 106 |
| Hoxd10 (NM_001206424.1) | R 5′ CCAGCGTTTGGTGCTTAGTGT 3′ | ||
| Gapdh | F 5′ AGGTCGGAGTGAACGGATTTG 3′ | 60 | 60 |
| Gapdh | R 5′ AGTTAAAAGCAGCCCTGGTGAC 3′ |