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Analysis of purified GCase extracted from root culture. (A) SDS-PAGE analysis, (B) Western blot analysis, and (C) GCase enzymatic activity. Primary antibody, anti-GCase from rabbit; secondary antibody, anti-rabbit-IgG, horseradish peroxidase (HRP)-linked. M, precision plus protein standards marker; +, Cerezyme®; WT, wild type; GC, GCase. Arrows indicate the GCase band (68 kDa).
