Table 1.
Composition of the N-glycan structure attached to purified GCase from leaves [24] and purified GCase from root culture.
| Structure | Amount of Composition (%) | |||||
|---|---|---|---|---|---|---|
| N270 | N146 | N59 | ||||
| GCWT | GCRoot | GCWT | GCRoot | GCWT | GCRoot | |
| FucGlcNAc2 | - | 1.1 | - | - | - | - |
| ManFucGlcNAc2 | - | 1.0 | - | - | - | - |
| Man2FucGlcNAc2 | 6.7 | 6.6 | - | - | - | - |
| Man3FucGlcNAc2 | 9.2 | 6.9 | 4.6 | 3.7 | 4.0 | - |
| Man2GlcNAc2 | - | - | - | 1.0 | - | - |
| GlcNAc2Man3XylFucGlcNAc2 | 5.2 | 6.6 | - | 6.2 | 7.2 | 8.5 |
| GlcNAcMan3XylFucGlcNAc2 | 10.0 | 10.1 | 5.2 | 12.6 | 19.5 | 16.7 |
| Man4XylFucGlcNAc2 | - | 0.4 | - | - | - | - |
| Man3XylFucGlcNAc2 | 43.5 | 43.3 | 65.5 | 56.8 | 56.1 | 58.7 |
| Man3XylGlcNAc2 | 1.5 | 1.6 | 5.5 | - | 1.8 | - |
| Man2XylFucGlcNAc2 | 23.9 | 19 | 16.5 | 17.7 | 11.3 | 16.1 |
| Man2XylGlcNAc2 | - | 0.8 | 2.7 | 2.0 | - | - |
| ManXylFucGlcNAc2 | - | 2.6 | - | - | - | - |
| 100 | 100 | 100 | 100 | 100 | 100 | |