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. 2018 Jun 29;19(7):1909. doi: 10.3390/ijms19071909

Figure 3.

Figure 3

The effects of CH-5 on the migratory and invasive ability of U2OS cells. (A,B) U2OS migration in wound healing assays. A confluent monolayer was wounded with a sterile pipette tip, and the cells were allowed to migrate for 24 h in the presence of DMSO (control) or CH-5 at the indicated concentrations for 24 h. CH-5 reduced the migratory ability of U2OS cells compared to control cells (* p < 0.05); (C) Migration of U2OS cells in Transwell assays; (D) Invasion of U2OS cells in Matrigel, in Transwell assays. In both assays, the cells were seeded in the top chamber and treated with DMSO (control) or CH-5. After 24 h, the cells that had invaded through the membrane were stained, photographed, and quantified (bar graph). The data are expressed as means ± S.E.M. (n  =  3); * p < 0.05 and ** p < 0.01 vs. no treatment; (E) CH-5 suppresses the expression of matrix metalloproteinase MMP-2 and MMP-9 in U2OS cells. The cells were treated with CH-5 at the indicated concentrations for 24 h and then subjected to zymography to analyze the activity of MMP-2/-9.