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. 2018 Jun 27;19(7):1892. doi: 10.3390/ijms19071892

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The α-Crystallin domain of CRYAB rescues Golgi localization of ATP7B-H1069Q mutant. (A) immunofluorescence analysis of COS7 cells transfected to express GFP-tagged ATP7B or ATP7B-H1069Q (ATP7B-H10) alone, or co-transfected to express full-length HA-tagged CRYAB or the isolated HA-tagged N-terminal (NTD), α-crystallin (ACD) and C-terminal (CTD) domains, as indicated on the left. After fixation, the cells were processed for triple confocal immunofluorescence analysis to reveal the Golgi complex with the marker GOLGA2. Note that the CTD was expressed at much lower level. White arrows point to region of GFP and GOLGA2 overlapping signal. Scale bar: 10 μm; (B) colocalization analysis of GFP and GOLGA2 signals (mean ± SD, n = 20 cells from two independent experiments). Abbreviations as in A.