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. 2018 Jun 27;19(7):1892. doi: 10.3390/ijms19071892

Figure 2.

Figure 2

Pept 73–92 specifically rescues Golgi localization of ATB7B-H1069Q. (A–G) immunofluorescence analysis of COS7 cells transfected to express GFP-tagged ATP7B or ATP7B-H1069Q alone, or co-transfected to express full-length HA-tagged CRYAB or incubated with TAMRA-tagged (T) or untagged Pept 73–92 or control peptide 1 (Ctrl Pept), as indicated in each panel. After fixation, the cells were processed for triple confocal immunofluorescence analysis to reveal the Golgi complex with the marker GOLGA2. White arrows as in Figure 1. Scale bar: 10 μm; (H) colocalization analysis of GFP and GOLGA2 signals (means ± SD, n = 15 cells from three independent experiments). (I) Quantification of the percentage of cells exhibiting exhibiting ER, Golgi complex, and both ER and Golgi complex GFP staining (means ± SD, n = 25 cells from two independent experiments). Abbreviations as in Figure 1.