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. 2018 Jun 27;19(7):1892. doi: 10.3390/ijms19071892

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FLIM-FRET analysis of the interaction between TAMRA-tagged Pept 73–92 and ATP7B-H1069Q. COS7 cells were transfected to express GFP-tagged ATP7B or ATP7B-H1069Q, incubated in the presence of TAMRA-tagged Pept 73–92 or control peptide 1 as in Figure 2, fixed and analyzed. (A) lifetime values τ of ATP7B and ATP7B-H1069Q in the presence or in the absence of the peptides; (B) percentage of FRET efficiency (percentage of ATP7B and ATP7B-H1069Q τ decrease in the presence of peptides). Means ± SD, n = 10 cells from 2 independent experiments.