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. 2018 Jun 29;19(7):1912. doi: 10.3390/ijms19071912

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Changes in endogenous melatonin and nitric oxide (NO) levels in response to NaCl stress. Three-day-old rapeseed seedlings were transferred to 200 mM NaCl for 2 days. Meanwhile, melatonin (A) detected by enzyme-linked immunosorbent assay; and NO contents (B) determined by visible spectrophotography, and (C) determined by laser confocal scanning microscopy, and expressed as relative fluorescence intensity) in seedling roots were analyzed. The sample without chemicals was the control (Con). Values are means ± SE of three independent experiments with at least three replicates for each.

Changes in endogenous melatonin and nitric oxide (NO) levels in response to NaCl stress. Three-day-old rapeseed seedlings were transferred to 200 mM NaCl for 2 days. Meanwhile, melatonin (A) detected by enzyme-linked immunosorbent assay; and NO contents (B) determined by visible spectrophotography, and (C) determined by laser confocal scanning microscopy, and expressed as relative fluorescence intensity) in seedling roots were analyzed. The sample without chemicals was the control (Con). Values are means ± SE of three independent experiments with at least three replicates for each.