Figure 8.

Melatonin modulated ion homeostasis via NO. Three-day-old rapeseed seedlings were pretreated with 1 μM melatonin, 10 μM SNP, 200 μM PTIO, alone or their combinations for 12 h, and then transferred to 200 mM NaCl for another 2 days (A) or 12 h (B,C). Afterwards, Na⁺ to K⁺ ratio (A) in seedling roots were detected by ICP-OES. The mRNA expression of NHX1 (B) and SOS2 (C) were analyzed by qPCR. The sample without chemicals was the control (Con). Values are means ± SE of three independent experiments with at least three replicates for each. Bars with different letters are significant different at p < 0.05 according to Duncan’s multiple range test.