Gene evidence indicated that SOS signaling pathway was regulated by melatonin via NO. Five-day-old wild-type (WT), noa1, and nia1/2 mutant plants were grown on MS medium supplemented with 1.0 μM melatonin for 5 days, and then transplanted in medium in the presence or absence of 125 mM NaCl for another 24 h. The mRNA expression of SOS1 (A), SOS2 (B), and SOS3 (C) in root tissues were analyzed by qPCR. Control seedlings (Con) were grown in MS medium alone. Data are means ± SE of three independent experiments with at least three replicates for each. Bars with different letters are significant different at p < 0.05 according to Duncan’s multiple range test.