Figure 3.

Effects of various blockers on the E2-evoked increase in the frequency of mPSCs in GnRH neurons in proestrus afternoon. The ERβ antagonist, PHTPP eliminated action of E2 in proestrus afternoon independently of whether PHTPP was applied extracellularly (A) or intracellularly (B). C, Intracellular application of the NO-scavenger CPTIO abolished the effect of E2 in proestrus afternoon. D, The Src inhibitor PP2 also eliminated the effect of E2. E, The PI3K blocker LY294002 also abolished the action of E2. F, Bar graph shows that effect of E2 was mediated via ERβ and the triggered retrograde NO signaling mechanism. The inserts below the 15 min recordings are 1-1 min zoomed periods from the respective recording before and after E2 administration. Individual PSC events are also shown from the control (to indentify in the 1-min zoomed periods, it is tagged by •) and the E2-treated (tagged by ▪) periods. Time course of the mean frequency and cumulative probabilities of the interevent intervals are presented under each recording.