. 2018 Aug 3;5(4):ENEURO.0057-18.2018. doi: 10.1523/ENEURO.0057-18.2018

Table 3.

Changes in mPSC parameters in GnRH neurons on E2 administration

Frequency of mPSCs	Frequency of control period (Hz)	Percentage change (%)	N/n	df	t	p
Pro pm E2	1.869 ± 0.1877	153.2 ± 17.33	12/7	11	3.067	0.0107^a
Pro am E2	1.074 ± 0.2078	110.2 ± 8.58	12/8	11	1.189	0.2593^a
Met pm E2	1.120 ± 0.2290	80.6 ± 7.54	9/6	8	2.591	0.0321^a
Pro pm PHTPP+E2	2.059 ± 0.2281	93.4 ± 9.07	7/4	6	0.7243	0.4962^a
Pro pm intraPHTPP+E2	1.790 ± 0.5129	97.1 ± 9.16	7/5	6	0.3222	0.7582^a
Pro pm CPTIO+E2	2.006 ± 0.2569	106.1 ± 5.93	9/5	8	1.027	0.3346^a
Pro pm PP2+E2	1.555 ± 0.2891	106.2 ± 6.06	8/6	7	1.014	0.3441^a
Pro pm LY294002+E2	1.703 ± 0.1989	102.2 ± 5.88	8/6	7	0.3805	0.7148^a
Pro pm Kynu+E2	1.279 ± 0.2357	133.7 ± 12.27	7/4	6	2.746	0.0335^a
Pro pm Picro+E2	0.631 ± 0.1376	143.1 ± 17.23	7/4	6	2.492	0.0470^a
Amplitude of mPSCs	Amplitude of control period (pA)	Percentage change (%)	N	df	t	p
Pro pm E2	-24.43 ± 2.067	100.7 ± 2.19	12/7	11	0.2760	0.7952^a
Pro am E2	-36.41 ± 5.148	101.5 ± 1.15	12/8	11	1.343	0.2062^a
Met pm E2	-34.51 ± 6.257	101.2 ± 2.84	9/6	8	0.4301	0.6785^a
Pro pm PHTPP+E2	-26.48 ± 3.562	97.0 ± 2.19	7/4	6	1.368	0.2203^a
Pro pm intraPHTPP+E2	-27.72 ± 8.614	99.1 ± 1.18	7/5	6	0.7337	0.4908^a
Pro pm CPTIO+E2	-25.05 ± 2.062	102.7 ± 2.192	9/5	8	1.216	0.2585^a
Pro pm PP2+E2	-26.46 ± 2.464	101.8 ± 3.84	8/6	7	0.4638	0.6569^a
Pro pm LY294002+E2	-36.90 ± 4.897	100.7 ± 1.18	8/6	7	0.5812	0.5793^a
Pro pm Kynu+E2	-36.83 ± 2.286	98.1 ± 2.37	7/4	6	0.7722	0.4693^a
Pro pm Picro+E2	-19.64 ± 2.107	97.0 ± 2.58	7/4	6	1.162	0.2894^a
Rise τ of mPSCs	Rise τ of control period (ms)	Percentage change (%)	N	df	t	p
Pro pm E2	3.941 ± 0.5402	138.8 ± 43.13	12/7	11	0.8985	0.3882^a
Pro am E2	4.287 ± 0.1746	134.3 ± 20.29	12/8	11	1.688	0.1194^a
Met pm E2	4.760 ± 0.6285	109.1 ± 16.41	9/6	8	0.5552	0.5939^a
Pro pm PHTPP+E2	3.710 ± 0.2690	103.3 ± 14.27	7/4	6	0.2302	0.8256^a
Pro pm intraPHTPP+E2	4.175 ± 0.9160	116.7 ± 24.64	7/5	6	0.6763	0.5241^a
Pro pm CPTIO+E2	3.771 ± 0.4979	143.9 ± 44.24	9/5	8	1.611	0.1459^a
Pro pm PP2+E2	3.970 ± 0.4466	99.1 ± 13.22	8/6	7	0.0657	0.9494^a
Pro pm LY294002+E2	3.708 ± 0.2870	92.6 ± 9.09	8/6	7	0.818	0.4403^a
Pro pm Kynu+E2	4.220 ± 0.4710	101.2 ± 14.99	7/4	6	0.0789	0.9396^a
Pro pm Picro+E2	3.006 ± 0.1134	124.4 ± 27.99	7/4	6	0.8729	0.4163^a
Decay τ of mPSCs	Decay τ of control period (ms)	Percentage change (%)	N	df	t	p
Pro pm E2	10.60 ± 2.416	137.2 ± 27.44	12/7	11	1.656	0.1259^a
Pro am E2	13.04 ± 5.393	130.8 ± 24.20	12/8	11	1.272	0.2297^a
Met pm E2	13.34 ± 1.805	141.4 ± 23.43	9/6	8	1.768	0.1150^a
Pro pm PHTPP+E2	13.44 ± 3.977	105.3 ± 22.41	7/4	6	0.2359	0.8214^a
Pro pm intraPHTPP+E2	11.83 ± 1.433	125.7 ± 16.45	7/5	6	1.562	0.1692^a
Pro pm CPTIO+E2	13.62 ± 2.368	113.4 ± 22.79	9/5	8	0.5898	0.5716^a
Pro pm PP2+E2	10.67 ± 1.033	94.5 ± 7.38	8/6	7	0.7455	0.4803^a
Pro pm LY294002+E2	13.45 ± 2.559	97.3 ± 9.51	8/6	7	0.2853	0.7837^a
Pro pm Kynu+E2	12.48 ± 1.152	103.5 ± 8.27	7/4	6	0.4269	0.6843^a
Pro pm Picro+E2	5.62 ± 1.101	79.3 ± 21.57	7/4	6	0.9602	0.3740^a

N = number of neurons measured. n = number of animals used for the given experiment.

df = degree of freedom of Student’s t test of percentage data where each neuron serves as its own control.

t = t values of Student’s t test of percentage data.

p = p probability values of Student’s t test of percentage data.

Pro = proestrus; Met = metestrus; am = morning; pm = afternoon.