Figure 2.

Identification of Syzygium simile leaf extract (SSLE) as an inhibitor of lipid accumulation. The platform described in Figure 1 was used for HTS. The screening flowchart is shown in Figure S2. (A) The XY-scatter plot shows the distribution of normalized LD count and area in primary screening. Each dot represents the mean of four fields from one well treated with one extract from the Taiwanese indigenous plant (TIP) library. Extracts that reduced LD formation by >40% (LD content <60%, which was approximately equivalent to <4.5 SDs (SD of all OA control wells); dashed lines) are indicated by red dots. Extracts that fitted the criteria without severe cytotoxicity (cell count >60%) were primary screen hits (blue dots). The results of re-validation are shown in Figure S3; (B,C) Huh7 cells were treated with BSA or OA with the indicated concentrations of SSLE or 1 μM TC for 24 h. (B) Representative images; (C) Quantification results. Bar: 50 μm. * significant difference from OA control group (** p < 0.01, *** p < 0.001, compared with controls using the one-way analysis of variance (ANOVA) and Dunnett’s tests).