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. 2018 Aug 3;15:26. doi: 10.1186/s12014-018-9202-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Effect of storage time on peptide yield. a Average peptide yield per sample at each RTR. b Total peptide extraction yield (µg) from SEER specimens versus specimen age, evaluated by BCA assay. Each point represents total yield from five 10-µm FFPE sections, scraped and pooled in a single tube for sample processing. Different colors are used for each RTR. c Peptide yield normalized to tumor volume (surface area × 0.01 mm depth) versus time in storage for the two RTRs reporting tumor dimensions