FIGURE 5.

RT-qPCR analysis of MBNL1 and MBNL2 gene and alternative splicing assay. (A) RT-qPCR assay for MBNL1 and MBNL2 genes expression in the HDFs to hiPSCs transition of WT and DM2 lines. Gene expression levels reported are an average of the WT and DM2 cell lines normalized to β-actin used as reference gene. (B) Eight-switch-like splicing changes of genes regulated by MBNL1 protein have been assessed by conventional RT-PCR analysis during the HDFs to hiPSCs transitions in WT and DM2 lines. The upper and lower bands on each gel represent the long (exon-included) and short (exon-omitted) isoforms, respectively.