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RNA-FISH in NPs. Representative confocal image of the analysis for intranuclear foci (red) and neuron marker β-tubulin 3 (TUJ1, green), dendritic marker (MAP2, green), and astrocytes marker GFAP (green) performed on DM2 and WT NPs. 4,6-Diamidino-2-phenylindole (DAPI) nuclear staining (blue). Image show the presence of CCTG RNA foci only in DM2 nuclei. Scale bars = 25 μm.
