Agarose gels showing the enzyme-digested patterns of PCR products of TNF-α −308 G/A. Electropherogram of ethidium bromide stained 4% agarose gel showing genotype pattern obtained with Ncol restriction digest at −308 G/A TNF-α polymorphism. Lane L represents 100-bp DNA ladder (Fermentas, Germany) while lane UD refers to the undigested PCR-amplified (134 bp) fragment. The other lanes refer to genotype pattern of samples. Genotypes of the samples are noted at the bottom of figure. Genotypes are characterized as GG (114 bp, 20 bp), GA (134 bp, 114 bp, and 20 bp), and AA (134 bp). On the gel, 20-bp fragments cannot be seen.