FIGURE 3.
UV-Vis absorptivity of ferulic acid, 4-vinylguaiacol (4-VG) and air. (A) Absorption spectra of wavelengths ranging from 250–400 nm of 1 mM ferulic acid (
), 1 mM 4-VG (
) and water (
) were determined in 96-well microtiter plates in a Tecan Infinite M200Pro spectrophotometer. The data presented show average ± standard deviation of eight independent replicate experiments. (B) Absorption spectra of wavelengths ranging from 250 nm to 400 nm of S. eubayanus CBS 12357 (SePAD1-SeFDC1/SePAD1-SeFDC1, Pof +) (
), IMK747 (Sepad1-Sefdc1Δ::amdSYM / SePAD1-SeFDC1, Pof +) (
)and IMK749 (Sepad1-Sefdc1Δ::amdSYM/Sepad1-Sefdc1Δ::amdSYM, Pof-) (
). Cells were grown in SWM-fa at 20°C for 72 h. Absorption spectra were determined of five times diluted supernatants in a Tecan Infinite M200Pro spectrophotometer.
