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. 2018 Jul 27;9:1640. doi: 10.3389/fmicb.2018.01640

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Copyright © 2018 Diderich, Weening, van den Broek, Pronk and Daran.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of UV variants expressing improved phenotype. (A) Cinnamic acid sensitivity assay. Five selected variants (HTSE022, HTSE023, HTSE033, HTSE037, and HTSE042) and control strains CBS1257, IMK747, and IMK749 were grown in 24-deepwell plates in 3 ml SMW and SMW-ca. Growth and final OD_660nm was recorded and differential growth was statistically evaluated by a Student’s t-test. Significance threshold was set at 0.01. The data presented show average ± standard deviation of three independent replicate experiments. (B) 4-VG concentration profiles of CBS 12357 (), IMK749 (), HTSE022 (), HTSE023 (), HTSE033 () cultivated in 24-deepwell plates in 3 mL SWM-fa. (C) 4-VG concentration profiles of CBS 12357 (), IMK749 (), HTSE037 (), HTSE042 () and S. pastorianus CBS1483 () grown in 24-deepwell plates in 3 mL SWM-fa. (D) Consumed ferulic acid in SWM-fa by CBS 12357, IMK749, HTSE022, HTSE23, and HTSE33. (E) Consumed ferulic acid in SWM-fa by CBS 12357, IMK749, HTSE037, HTSE042 and S. pastorianus CBS 1483. The colored code used in panels D and E is identical to that used in panels (D) and (E). The data presented show average ± standard deviation of independent triplicate experiments.

Inline graphic — Characterization of UV variants expressing improved phenotype. (A) Cinnamic acid sensitivity assay. Five selected variants (HTSE022, HTSE023, HTSE033, HTSE037, and HTSE042) and control strains CBS1257, IMK747, and IMK749 were grown in 24-deepwell plates in 3 ml SMW and SMW-ca. Growth and final OD_660nm was recorded and differential growth was statistically evaluated by a Student’s t-test. Significance threshold was set at 0.01. The data presented show average ± standard deviation of three independent replicate experiments. (B) 4-VG concentration profiles of CBS 12357 (), IMK749 (), HTSE022 (), HTSE023 (), HTSE033 () cultivated in 24-deepwell plates in 3 mL SWM-fa. (C) 4-VG concentration profiles of CBS 12357 (), IMK749 (), HTSE037 (), HTSE042 () and S. pastorianus CBS1483 () grown in 24-deepwell plates in 3 mL SWM-fa. (D) Consumed ferulic acid in SWM-fa by CBS 12357, IMK749, HTSE022, HTSE23, and HTSE33. (E) Consumed ferulic acid in SWM-fa by CBS 12357, IMK749, HTSE037, HTSE042 and S. pastorianus CBS 1483. The colored code used in panels D and E is identical to that used in panels (D) and (E). The data presented show average ± standard deviation of independent triplicate experiments.