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. 2018 Jul 27;9:1640. doi: 10.3389/fmicb.2018.01640

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Copyright © 2018 Diderich, Weening, van den Broek, Pronk and Daran.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sequence comparison of parental strain S. eubayanus CBS 12357 with UV-mutagenized variant HTSE042. Sequencing read mapping of S. eubayanus reference CBS 12357 and UV-variant HTSE042 strain onto CBS 12357 assembly (PRJNA243390) (Baker et al., 2015). The figure represents local mapping at the end of right arm of S. eubayanus CHRXIII. Both strains showed a similar coverage of the region (ca 95-100-fold). HTSE042 showed an absence of read mapping at the chromosomal region between S. eubayanus ortholog of S. cerevisiae YNR073C renamed SeYNR073C and SeVBA5 located at the end of SeCHRXIII. Reads were mapped using Burrows-Wheeler Aligner BWA (Li and Durbin, 2010). The resulting bam files were visualized using IGV (http://software.broadinstitute.org/software/igv/).