Table 3.
CASI and FACI performance indicators of the parental S. eubayanus strain CBS 12357, of single and double PAD1-FDC1 deletion mutants IMK747 and 749, and of five UV-mutagenized isolates.
| S. eubayanus strain | Relevant characteristics | CASI | FACI |
|---|---|---|---|
| CBS 12357 | MATa/MATα SePAD1-SeFDC1/SePAD1-SeFDC1) Pof+ | 0.1 | 72.7 |
| IMK747 | MATa/MATα Sepad1-Sefdc1Δ::amdS/SePAD1-SeFDC1 Pof+ | 22.7 | 36.7 |
| IMK749 | MATa/MATα Sepad1-Sefdc1Δ::amdS/Sepad1-Sefdc1Δ::amdS Pof- | 72.7 | 15.7 |
| HTSE022 | MATa/MATα SePAD1-SeFDC1/SePAD1-SeFDC1 (UV mutagenised) Pof+ | 11.8 | 59.9 |
| HTSE023 | MATa/MATα SePAD1-SeFDC1/SePAD1-SeFDC1 (UV mutagenised) Pof+ | 5.7 | 51.9 |
| HTSE033 | MATa/MATα SePAD1-SeFDC1/SePAD1-SeFDC1 (UV mutagenised) Pof+ | 12.7 | 42.9 |
| HTSE037 | MATa/MATα SePAD1-SeFDC1/SePAD1-SeFDC1 (UV mutagenised) Pof- | 60.6 | 15.7 |
| HTSE042 | MATa/MATα Sepad1-Sefdc1Δ/ Sepad1-Sefdc1Δ∗ (UV mutagenised) Pof- | 68.3 | 15.4 |
The UV mutagenized isolates (HTSE022-023, HTSE033, 37 and 42) were grown in 96-well microtiter plates filled with SWM at 20°C for 48 h in an orbital incubator (Infors Multitron) at 250 rpm. Subsequently, the microtiter plates were replica-plated in three different media by transferring 10 μl of each culture into fresh microtiter plates filled with either 200 μL SWM or SWM-fa or SWM-ca. The reference strain S. eubayanus CBS 12357, IMK747 and 749 were grown in shake flask filled with either 100 mL SWM or SWM-fa or SWM-ca. CASI and FACI indicators were calculated as described in the Materials and Methods section. ∗Indicates pad1-fdc1 deletion resulting from UV mutagenesis.